The norovirus NS3 protein is a dynamic lipid- and microtubule-associated protein involved in viral RNA replication

BT Cotton; JL Hyde; ST Sarvestani; SV Sosnovtsev; KY Green; PA White; JM Mackenzie

Journal article

The norovirus NS3 protein is a dynamic lipid- and microtubule-associated protein involved in viral RNA replication

BT Cotton, JL Hyde, ST Sarvestani, SV Sosnovtsev, KY Green, PA White, JM Mackenzie

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2017

DOI: 10.1128/JVI.02138-16

Abstract

Norovirus (NoV) infections are a significant health burden to society, yet the lack of reliable tissue culture systems has hampered the development of appropriate antiviral therapies. Here we show that the NoV NS3 protein, derived from murine NoV (MNV), is intimately associated with the MNV replication complex and the viral replication intermediate double-stranded RNA (dsRNA). We observed that when expressed individually, MNV NS3 and NS3 encoded by human Norwalk virus (NV) induced the formation of distinct vesicle-like structures that did not colocalize with any particular protein markers to cellular organelles but localized to cellular membranes, in particular those with a high cholesterol ..

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University of Melbourne Researchers

Jason Mackenzie Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

We thank Stephen Robbins (University of Calgary, Canada) for providing the stomatin and flotillin expression plasmids. We acknowledge the facilities and the scientific and technical assistance of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, University of Queensland, and the University of Melbourne's Biological Optical Microscopy Platform (BOMP). Project grants (no. 1010327 and 1083139) awarded to J.M.M. and P.A.W. from the National Health and Medical Council of Australia supported this research.